The use of restriction enzymes is evidenced by the statement 'cut site mCYCF3E 685' (Option A).
The restriction enzymes are proteins (enzymes) that cut specific sites in the nucleotide sequence.
In this case, the enzyme cuts the gene mCYCF3E at the position number 685 (nucleotide base).
In conclusion, the use of restriction enzymes is evidenced by the statement 'cut site mCYCF3E 685' (Option A).
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