Restriction enzymes cleaves the plasmid DNA and ligase enzyme seals the selected part of DNA and plasmid vector for gene cloning to produce certain proteins
restriction nucleases' cleavage of DNA at certain locations, considerably facilitating the separation and modification of individual genes. When a single DNA molecule is cloned to create many billions of identical molecules, this process is known as DNA cloning. It can be done via cloning vectors or the polymerase chain reaction. The capacity of a particular DNA or RNA sequence to bind to a complementary nucleic acid sequence allows for nucleic acid hybridization
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