Nature, allolactose serves as the inducer in the wild-type lac operon. for this experiment, you used IPTG as the inducer. It is so important to our experiment that iptg is not metabolized the bacterial cells can't process IPTG since it is not the right substrate for the lactose metabolic pathways.
what is allolactose ?
A disaccharide like lactose is allolactose. Instead of the 1-4 linkage of lactose, it consists of the monosaccharides D-galactose and D-glucose joined through a 1-6 glycosidic linkage.
what is IPTG ?
A molecular biology tool is isopropyl d-1-thiogalactopyranoside. This substance is utilized to promote protein expression in situations where the lac operator controls the gene because it is a chemical mimic of allolactose.
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