The best method for enumerating microbes present in low density environment, such as a pond, would be Most Probable Number method.
Bacteria is one of the microorganisms that are capable of contaminating food and water bodies such as ponds and lakes.
The measurement of bacterial growth can be used to estimate the number of bacterial cells in a given sample from either food, water or specimen from living organism.
The pond is known to be a low density environment for microorganisms because the quantity of organisms gotten from this sample are usually low, therefore Most Probable Number method is used.
The Most Probable Number method evaluates detectable growth by observing changes in turbidity or color due to metabolic activity.
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