You need to make a 1:4 dilution of dye in water so that the total volume in test tube one is 60 ml.

From the solution you made in test tube one, you need to make a 1:5 dilution into test tube two so that the total volume is 15 ml.

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You need to make a 14 dilution of dye in water so that the total volume in test tube one is 60 ml From the solution you made in test tube one you need to make a class=

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In the serial dilution for the preparation of the dye, the total dilution factor is 1/20

What is dilution of solution?

Dilution is the process of preparing solutions of lesser concentration from a more concentrated solution.

In test tube 1:

Total volume = 60 mL

Dilution factor = 1:4

1 parts stock solution is required for every four parts diluent

Total parts = 5

volume of diluent = 4/5 × 60 mL = 48 mL

48 mL of diluent is added to 12 mL of stock solution to prepare the solution in test tube 1.

In test tube 2:

Total volume = 15 mL

Dilution factor = 1:5

1 parts stock solution is required for every five parts diluent

Total parts = 6

volume of diluent = 5/6 × 15 mL = 12.5 mL

12.5 mL of diluent is added to 2.5 mL of stock solution from tube 1 to prepare the solution in test tube 2.

Total dilution factor = 1/4 × 1/5 = 1/20

Learn more about dilution at: https://brainly.com/question/24881505