The pH of the TRIS acid is [tex]8.1 + log [\frac{A-}{0.1} ][/tex].
The pH of the TRIS base is [tex]8.1 + log [\frac{B}{0.1} ][/tex].
pH of the TRIS acid
The pH of the TRIS acid is determined by applying Henderson - Hasselbalch equation as follows;
[tex]pH=pK_a + log \ [\frac{A^-}{HA} ][/tex]
where;
pKa of TRIS acid = 8.1
[tex]pH = 8.1 + log [\frac{A-}{0.1} ][/tex]
The pH of the TRIS base is determined by applying Henderson - Hasselbalch equation as follows;
[tex]pH=pK_a + log \ [\frac{B}{BH^+} ][/tex]
where;
[tex]pH = 8.1 + log [\frac{B}{0.1} ][/tex]
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