RNAi-based modification of amylopectin content in wheat occurs through decreased expression of starch-branching enzymes. If the siRNA used to knock down plant starch-branching enzymes was transmitted to humans, what enzyme might it affect, and why would this be harmful

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espiritu2072 espiritu2072

03-12-2021
Biology

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RNAi-based modification of amylopectin content in wheat occurs through decreased expression of starch-branching enzymes. If the siRNA used to knock down plant starch-branching enzymes was transmitted to humans, what enzyme might it affect, and why would this be harmful

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luisvaschetto luisvaschetto · Answer 1 · 2021-12-06T11:28:26+01:00

If siRNA against a starch-branching enzyme was transmitted to humans, then it may affect the expression of glycogen-branching enzymes. RNAi inhibits gene expression.

Glycogen-branching enzymes are similar to starch-branching enzymes because glycogen bonds are similar to those observed between amylopectin.

The RNA interference (RNAi) pathway is an evolutionarily conserved mechanism used in molecular biology laboratories to inhibit the expression of target genes.

In the RNAi technique, a regulatory non-coding RNA called small interfering RNA (siRNA) that exhibits sequence complementary to the target gene sequence is used to inhibit and/or block the translation of the target mRNA (in this case, starch/glycogen-branching mRNA coding enzyme).

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