Mainly, glucose such as monosaccharide give positive Benedict's test.
What is Benedict's test?
- The Benedict's test uses for identification of reducing sugar.
- The sugar may be monosaccharide are disaccharides.
- This test is given in alkaline medium.
- The reagent used in Benedict's test is complex mixture of sodium citrate, sodium carbonate and the pentahydrate of copper(II) sulfate. This reagent is called Benedict's reagent.
Write the chemical reaction equation for that positive Benedict's test showing how the structure of the monosaccharide changed during the test.
In Benedict's test, the sodium carbonate converts glucose into enediol and enediol is reduces cupric into cuprous forming cuprous hydroxide.
On boiling the solution which is kept in sodium citrate give red precipitate of cuprous oxide.The chemical reaction is shown below.
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