Answer:
The basic difference between SEM and TEM is that TEM generates an image with the assistance of radiation that goes through the specimen, while the images generated by the SEM take place with the assistance of electrons getting released from the surface of the specimen.
SEM is primarily used to examine the composition of the surface, with the help of SEM one can study the microbes present in the ecological niche like in the lining of the gut and human skin. Due to its great depth, it is considered the electron microscope analog of a stereo light microscope. It helps in providing complete images of the cells present on the surface, which are not easy to get with the help of TEM. Due to its enhanced functionality, it can also be used for size determination and particle counting, and also for process control.