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A student wants to use western blotting to confirm the presence of human Sonic hedgehog (Shh) in samples run on SDS-PAGE and transfered to a nitrocellulose membrane. The antibody reagent box in their lab contains Mouse anti-Shh MAb and Chicken anti-mouse IgG-HRP. What substrate will they need in order to perform colorimetric detection of Shh on their western blot

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ogorwyne

Answer:

BCIP/NBT

Explanation:

BCIP/NBT is a colorogenic substrate used for AP. AP stands for alkaline phosphatase. When they are catalyzed with this alkaline, we would have a product that has a dark blue or probably a purple color. NBT stands for nitro blue tetrazolium. Both of these work as substrate for the reaction. BCIP oxidizes BCIP to form this color.

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