Other than solubility of sample in the solvent, what is the criteria for the solvent (other than cheap and nontoxic) used for preparing the sample that impacts the experiment and why would this criteria be important in the spotting phase if you have to make repeated applications of the sample?

In order for the spotting phase to be efficient and for the sample to register the repeated applications of the sample, it is necessary that in addition to the solubility and low toxicity, the solvent has a low boiling point, but that allows an efficient and rapid evaporation, whenever necessary, so that it is possible to release the sample on the TLC plate.

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pstnonsonjoku pstnonsonjoku · Answer 2 · 2022-02-24T11:51:08+01:00

The solvent must be able to dissolve the compounds completely with minimal heating.

Even though the question is incomplete but I will try to help you as much as possible. Solvents are considered based .on their specific applications. Generally, a solvent should be non toxic and cheap.

Also, the solvent must be able to dissolve the compounds completely with minimal heating. This is important in deciding weather the solvent is suitable or not.

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