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In the "all or none" law of muscle contraction, it states that when the stimulus applied exceeds threshold then the the nerve sending signals to a few muscle fibers will give a complete response; contraction. Graded response is dependent on how many motor units have been recruited. The more motor units, the greater the force in the contraction.
The primer a scientist chooses will help target a specific region of the DNA molecule. Moreover, the temperature chosen in the annealing step of the polymerase chain reaction should be low enough for primer hybridization but high enough for the hybridization to be specifc.