Answer:
The correct answer is:
after the vein is entered (A.)
Explanation:
The procedure in the question describes venopuncture.
Venopuncture is the method of aseptically collecting blood into the right sample containers. Using a stopper-punturing needle and a vacuum tube, the following procedure is followed:
- assemble the needle and hub (needle holder)
- apply the tourniquet and examine the arm for palpable vein, palpate the vein
- release the tourniquet and swab the collection site with 70% alcohol
- allow the swab to dry
- reapply the tourniquet
- insert the stopper-puncturing needle (attached to hub) into the appropriate vein
- after the vein is entered, the stopper-puncturing needle should be completely pushed into the evacuated (vacuum) tubes
- blood will flow into the tube until vacuum is completely filled
- pull out the tube and invert gently several times if the tube contains anticoagulants
- place a gauze on the site of puncture (do not press down the gauze)
- remove the needle and press down the gauze immediately
- dispose the needle into the sharps container or appropriately
- label the tubes.