Answer:
PCR
Produces million of copies of a segments of DNA and
Requires cycles of temperature.
PCR.
When large number of copies of a particular gene fragment is needed from smallest quantity of DNA molecules,the techniques of genetic engineering usually employed is called PCR. It involves denaturation of template DNA,the use of Primers(20 base pairs),the use of enzyme DNA polymerase for building of new strands of DNA.
Natural Replication.
produces a copy of the whole genome
It occurs before mitosis and meiosis
Begins at the origin of replication.
Both.
Requires primers
Requires DNA polymerase
Results in Semi conservative replication
Explanation:
When rapid production of large copies of particular DNA fragments is needed from a small molecules of fragment of the original DNA fragments is needed the tool of genetic engineering used is called PCR.
It involves denaturation of the template DNA at 95 degrees for separation to single strands,
the attachments of primers(20 base pairs of complementary bases to the template DNA) at 65 degree a process called annealing and the use of polymerase enzyme for building on chains of DNA , during elongation.
The enzyme are extracted from thermophilic organisms,therefore could withstand the high temperature.The polymerization can proceed for a continuous cycle with million of new DNA chains produced by the polymerization Chain machine