Respuesta :
Primers
Explanation:
Primer refers to a small set of nucleotides of DNA, typically 18 to 24 base pairs in length
- In the PCR method, a pair of primers is used to hybridize with the sample DNA and define the region of the DNA that will be amplified
- Primers are also referred to as oligonucleotides
- Validated primers are crucial in determining the specificity, sensitivity and robustness of a PCR reaction
- The critical variable for primer performance is its annealing temperature rather than its melting temperature as the annealing temperature defines the temperature at which the maximum amount of primer is bound to its target
- qPCR assays generally use symmetric primers
- Because reannealing of the template strands gradually outcompetes primer and probe binding to the template strands and sequesters the polymerase
- This is a particular problem when the aim is to detect specific DNA targets down to alleles of single-copy genes in single cells
- Asymmetric amplification can be much less efficient and requires extensive optimization to identify the proper primer ratios, the amounts of starting material, and the number of amplification cycles that can generate reasonable amounts of product for individual template/target combinations
Answer:
The main property of primers is that they must correspond to sequences on the template molecule (must be complementary to template strand). Primers can be found in the STR region of the DNA. They attach to and surround new DNA nucleotides.
