Answer:
D) the synthesis of ATP
Explanation:
The chloroplast possesses the thylakoids and stroma in which the thylakoid is associated with the light reaction and ATP synthesis and the stroma is associated with the Calvin cycle.
The membrane of the thylakoid is involved in maintaining the membrane potential in which ATP synthase are embedded. The F1 unit of the ATP synthase or rotor rotates when the Hydrogen moves from the thylakoid membrane to the stroma.
If the membrane is punctured then it will disrupt the proton flow and process of ATP synthesis is disrupted.
Thus, Option-D is correct.