Scientists are testing substance L to determine how it enters mammalian cells in a culture. The cells maintain a 120 millimolar (mM) intracellular concentration of substance L. The scientists determined the rate of entry of substance L into the cells at various external concentrations of substance L (10 to 100 mM) in culture medium (Table 1). Table 1. Rate of entry of substance L into mammalian cells in culture External concentration of substance L (mM) Rate of entry of substance L into cell as a percent of maximum 10 5% 20 25% 30 45% 40 65% 50 80% 60 90% 70 95% 80 100% 90 100% 100 100% The cells maintain substance L at an internal concentration of 120 mM. (C) Predict the likely effect on the ability of substance L to enter the cells of substance L is attached to a large protein instead of free in the culture.

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Scientists are testing substance L to determine how it enters mammalian cells in a culture. The cells maintain a 120 millimolar (mM) intracellular concentration of substance L. The scientists determined the rate of entry of substance L into the cells at various external concentrations of substance L (10 to 100 mM) in culture medium (Table 1). Table 1. Rate of entry of substance L into mammalian cells in culture External concentration of substance L (mM) Rate of entry of substance L into cell as a percent of maximum 10 5% 20 25% 30 45% 40 65% 50 80% 60 90% 70 95% 80 100% 90 100% 100 100% The cells maiN

Scientists are testing substance L to determine how it enters mammalian cells in a culture. The cells maintain a 120 millimolar (mM) intracellular concentration of substance L. To predict the likely effect on the ability of substance L to enter the cells of substance L. Suppose substance L is attached to a large protein, the large protein will clearly decrease and reduce the rate of entry of substance L due to the fact that its weight increasing.

We can see from the data provided that as the external concentration of substance L increases, the rate of entry of substance L also gradually increases.

The movement of transport of substance L is called active transport since it moves from a region of lower concentration to higher concentration.

Substance L can move into the cell via active transport. However, this active transport is inversely proportional to molecular weight. As such, if substance L is attached to a large protein instead of free in the culture, the large protein will definitely decrease and reduce the rate of entry of substance L due to the fact that its weight increasing.

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