Answer: the introduction of characters and setting.
A conclusion is the last section of a narrative. It can contain various types of language and devices.
For example, conclusions often present the resolution of the conflicts present in the rest of the text. It can also serve as a space for the characters or the narrator to reflect on the events that they have just experienced. Finally, it can also serve as a cliff hanger, which means that there is no clear resolution and in fact something exciting or surprising has happened. This is commonly used to maintain the reader's interest in the narrative alive.
However, a conclusion cannot end with the introduction of new characters or settings. Because this is the last section, these introductions would be underdeveloped, which would diminish the quality of the narrative.